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Authors: Ekhaise, Frederick Osaro
Oronsaye, Ivie
Keywords: Ekenhuan Benin City
borehole water
bacteriological and physicochemical parameters.
Issue Date: 2012
Publisher: Journal of Life and Environmental Sciences
Series/Report no.: Vol. 13;1&2
Abstract: The bacteriological and physicochemical studies of borehole water samples from selected sites in Ekehuan Area, Benin-City were studied between June, 2011 and November, 2011 to ascertain the quality of water samples. The water samples were collected from three (3) different borehole sites which includes, properly sited borehole, borehole sited close to a refuse dumpsite, and a borehole sited close to erosion threatened area. The total heterotrophic bacterial counts ranged from 1 x 102 - 8.0 x l02cfu/ml from the months of June, 2011 - November, 2011. The bacterial counts were shown to be highest in water samples collected from borehole sited close to a refuse dumpsite which ranged from 2.5x102 - 8.0 x 102cfu/ml and a mean value of 5.02x102 ±0.87cfu/ml while the lowest counts were recorded for water samples collected from properly sited borehole which had a range of 1 x 102 - 2.0x102 cfu/ml with a mean value of 1.0x102 ±0.32cfu/ml Water sample collected from borehole sited close to erosion threatened area had bacterial counts ranged from 2.1 x 102 - 3.l x l02cfu/ml with a mean value of 2.22 x 102 ±0.47cfu/ml. The total coliform counts of water samples ranged from 0 MPN - 17 MPN/l00ml. The coliform counts recorded nil for water samples collected from properly sited borehole while the highest counts were recorded at borehole sited close to a refuse dumpsite with a value of I7MPN/100ml Culturally, morphologically, and biochemically, four bacterial isolates were isolated and characterized to belong to Pseudomonas aeruginosa, Bacillus sp., Klebslella sp., and Enlerobacte aerogenes. Among the bacterial isolates, Pseudomonas aeruginosa and Enterobacetr aerogenes were observed to be the most frequently isolated isolates with frequency of occurrence at 37.5% and 28.12% respectively. The antibiotics sensitivity testing showed that most isolates were sensitive to Ofloxacin (96.8%), Amoxicillin (50%), and Gentamycin (68.8%), while they were more resistant to Septrin (3.13%) and Tetracycline (6.25%). Plasmid profiling was carried out on bacterial isolates using agarose gel electrophoresis for extraction of plasmid DNA, which showed the presence of 1, 2, and 3 plasmids in the range 20 to 100kb. The aesthetic properties of the water samples were acceptable. However, water samples from boreholes sited close to refused dumpsite recorded higher values, which were above the approved standard limit of most physicochemical parameters, with ranges of 26.52±0.620C, 5.58±1.02Mg/l, 579.64±l6.3μscm-1, 153.67±l6.68Mg/l, 7.88±0.7Mg/I, 4.81±0.68Mg/I, 0.83±0.29Mg/I for temperature, BOD5, conductivity, total solids dissolved oxygen, nitrate and iron respectively. Analysis of variance (ANOVA) showed that there was a significant difference (P<0.01) in total heterotrophic bacterial counts and total coliform counts in all the water samples studied with water samples from borehole sited close to refuse dumpsite showing the significant difference. The studyrevealed the importance of good water managements to prevent outbreak of water related diseases.
ISSN: 119-9792
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