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Title: Microbiological and Physicochemical Analyses of Oil Contaminated Soil From Major Motor Mechanic Workshops In Benin City Metropolis, Edo State, Nigeria
Authors: EKHAISE, F O
Keywords: Oil contaminated soil
microbial isolates
mechanical workshops and physicochemical parameters
Issue Date: Dec-2011
Publisher: Journal of Applied Science and Environmental Management
Series/Report no.: Vol. 15 (4);597- 600
Abstract: The ability of microorganisms to utilize used oil in contaminated soil from some selected major mechanic workshops in Benin City metropolis as sole sources ofcarbon and energy was studied. Soil samples collected from the three major mechanic workshops at lgun, Evbareke and Uwelu Quarters in Benin City were analyzed for the microbiological and physicochemical qualities using the basic microbiological methods. The total heterotrophic bacterial counts from the three mechanic workshops ranges from 1.98 x 107 to 2.95x107cfu/g while the total heterotrophic funga1 counts ranges from 6.7 x106 to 9.2x106cfu/g. The hydrocarbon utilizing microbial isolates were isolated using the Bushnell Haas enrichment techniques. The hydrocarbon utilizing microbial populations were recorded to range from 3.1 x l06 to 9.7 x 106 cfu/g and 3.4 x 106 to 6.0x l06 cfu/g for the bacterial and fungal counts respectively. The bacterial isolates include the genera Bacillus, Pseudomonas, Micrococcus Flavobacterium and Klebsiella, while the fungal isolates include the genera Aspergillus, Penicillium, Fusarium, Trichodernma and Yeast. The biodegradation potentials of the hydrocarbon degrading microbial isolates monitored by gas chromatography (GC) showedPseudomonas sp. and Aspergillus versicolor to have recorded the highest biodegradation potentials among the microbial isolates. The frequency of occurrence of the microbial isolates revealed the microbial isolates Pseudomonas sp. Bacillus sp., Microococcus sp., Aspergillus versicolor. Trichoderma sp. and Penicillium sp. as the most frequently occurring isolates. The physicochemical properties of the soil samples analysed shows the pH ranges from 5.63 to 6.01, percentage of carbon 2.345 to 4.800, total organic matter 1.59 to 7.81%, total petroleum hydrocarbon 86.10 to 286.50 mg/kg and sulphate 1.49 to 6.10ppm. The correlation analysis showed that there was significant difference in the mean values for both total heterotrophic bacterial and fungal population among the locations. It was also showed that there exist significant difference in the optical density and pH among the microbial isolates.
ISSN: 1119-8362.
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